Background Hard mineral and acid salt deposits that clump together in concentrated urine are known as kidney stones. A complicated chain of physicochemical events, including hyper saturation, nucleation, growth, aggregation, and retention in the urinary tract, leads to urolithiasis, the third most prevalent urinary tract issue. Numerous traditional medications and therapies are less successful in treating urolithiasis and frequently cause recurrences. Objective The present study is focused on evaluating the anti-urolithiasis activities of the polyherbal formulation prepared from Spinacia oleracea L. and Coriandrum sativum L. seeds. Methods To evaluating the anti-urolithiasis activities of the polyherbal formulation prepared from S. oleracea and C. sativum seeds against oxalate crystals by employing an in vitro inhibition, aggregation, and nucleation assay, employing a spectrophotometer followed by microscopic observation, and further monitored by egg semipermeable membrane model, using cystone as a standard drug. Results In the present work, the alkaloids, glycosides, flavonoids, glycosides, phenols, saponins, steroids, tannins, terpenoids, and triterpenoids were found in the extracts of S. oleracea and C. sativum. The in vitro assays in the present study clearly indicated that all the mixture extracts readily prevented crystal nucleation, growth and aggregation. The aqueous extract of the mixture inhibited significant inhibitory potential against the crystallization process. Conclusion It can be said that the phyto-constituents enriched homemade preparation possibly will be used as a medication for the management of kidney stones by dissolving the oxalate stone in the kidney.